Content: An exploration of ecology and evolution. To foster a sense of wonder and curiosity about biological diversity. To develop skills in oral communication, use of the computer as a scientific tool, and functioning as a member of a goal-directed team. To gain experience in the practice of science by posing research questions, designing and conducting experiments or observations to answer these questions and presenting the results publicly. To introduce ecological and evolutionary principles, and how these relate to understanding the origins and diversity of life on earth. Our data confirm the potential of ITS region PCR-RFLP for the study of polymorphism amongTrichoderma population from different soil layers.Abstract Goals: This course is designed for potential biology majors and others needing majors-level biology. Thus the RFLP analysis produced different DNA profiles on the gels denoting significant intraspecific genetic variation. A high degree of polymorphism was detected in rhizosphere rather than soil. The restriction profiles of the PCR products showed thatHaeIII enzyme has more discriminatory power thanRsaI. Amplification products showed extensive length polymorphism and RFLP analysis generated bands ranging from 100 to 620 bp. The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with two restriction enzymes. The diversity ofTrichoderma between soil horizons was evaluated by analysis of the internal transcribed spacer (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). One hundred-five isolates obtained were identified at the genus level by analysis of morphological characters. We investigated the occurrence ofTrichoderma in the Oueslatia forest located in the centre-west of Tunisia, which represents a good example for studying a distribution ofTrichoderma between different soil horizons of a forest with Mediterranean bioclimate. Conclusions: PCR-RFLP is a highly sensitive, specifc, and direct method for fungal detec- tion and can be used for fungal epidemiological studies in HIV-positive and other im- munocompromised patients. albicans was the most commonly identifed species (82.2%), followed by C. Results: We successfully identifed the diferent Candida spp. Universal primers for the internal transcribed spacer (ITS) region (ITS1–ITS4) of the fungal rRNA genes were used for this assay. Patients and Methods: We identifed 96 Candida isolates obtained from 139 Iranian pa- tients infected with the human immunodefciency virus (HIV), between April 2009 and April 2010, by using PCR-RFLP assay. isolated from the oral cavities of HIV-infected patients in southeastern Iran (Kerman), by using PCR-based re- striction enzyme digestion. Objectives: The purpose of this study was to identify Candida spp. Polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP) is a rapid, sensitive, and specifc method for detection of clinically important fungi. Rapid identifcation of candidiasis is important for the clinical management of immunocompromised patients. Background: The incidence of opportunistic infections due to Candida albicans and other Candida spp.
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